Archive

Microbial load in unpasteurized milk obtained from selected milk outlets in Kawempe division of Kampala, Uganda

John N Kateregga; Collins Atuheire; Esther Nabatta; Samuel Majalija; James G Ndukui*

    Background: Milk consumption and demand has increased drastically over the past years in response to expanding human population in Uganda and world at large. Despite this, many residents of Kampala city obtain milk from shops supplied by milk vendors, who in turn, collect it directly from farms. The microbial load of this unpasteurized milk is not known, yet it could expose the population to milk-borne diseases..


Detection of antibodies anti-gp43 of Paracoccidioides brasiliensis in sera samples by double-sandwich ELISA

Luís Felipe Minozzo Figueiredo; Barbara Ribeiro Fonseca-de-Souza; Alessandra Becker-Finco; Elizângela Almeida Rocha; Luísa Lasmar Lima Oliveira; Ricardo Machado-de-Ávila; João Carlos Minozzo; Fernanda Costal-Oliveira; Larissa Magalhães Alvarenga; Vanete Soccol; Carlos Chávez-Olórtegui*

    Monoclonal antibodies (mAbs) were raised against 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis. One mAb anti-gp43 and gp43 were used in a double-antibody sandwich ELISA to detect antibodies in sera from patients with Paracoccidioidomycosis. This ELISA diagnosis showed 91.18% sensibility and 100.0% specificity against sera of patients with histoplasmosis, aspergillosis or chromomycosis.


Effect of antiretroviral drugs on the renal function of HIV seropositive patients attending ISS clinic of Mulago Hospital, Uganda

John N Kateregga; Collins Atuheire; Justine Nalunga; Ndukui G James*

    Background: Highly Active Antiretroviral Therapy (HAART) consisting of at least three active drugs against HIV infection has improved HIV/AIDS management since their inception. However, alterations of lipid metabolism due to HAART can result into secondary diabetic and hypertensive renal damage. This study was carried out to assess the effect of antiretroviral drugs on the renal function of HIV seropositive patients by determining the serum levels of Creatinine, Urea and Electrolytes (Na, K, and Cl) of the patients on treatment.


Anti-aging effects of the proteins from artemia extract on human fibroblasts cell proliferation and collagen expression in induced aging conditions

Neda Vaseli-Hagh; Abdolkhaleg Deezagi*; Mahvash Khodabandeh Shahraki

    Researches on the factors that inhibit senescence are attractive in health industries. Artemia extract uses in cosmetic and sunlight protecting products. The aim of this work was to study the effects of Artemia extract on the cell proliferation, senescence and collagen synthesis by human fibroblasts in normal and aging induced conditions.


Somatic mutations in IFN-γ-Signal molecules in human uterine leiomyosarcoma

Takuma Hayashi*; Tomoyuki Ichimura; Mari Kasai; Kenji Sano; Susumu Tonegawa; Yae Kanai; Dorit Zharhary; Hiroyuki Aburatani; Nobuo Yaegashi; Ikuo Konishi

    Uterus Leiomyosarcoma (uLMS), unfortunately, is a disease with a poor prognosis. In the report of the Ministry of Health, Labor and Welfare, the 50% survival time (mOS) summarized from Stage I to Stage IV was 31months. Preoperative diagnosis of uLMS is very difficult. Although it is diagnosed as "uterine fibroids", it is not uncommon in cases where the uterine leiomyosarcoma is definitely diagnosed again after surgical hysterectomy or removal of myoma.


Modern biotechnology: Origination of paper-based analytical devices

Shilpa Sivashankar*; Prasad Salunke

    Nowadays most of the biotechnological processes can be performed on benchtop platforms without the need for an expensive, space-consuming equipment. In addition, the sensitivity and specificity of the conventional assays using biotechnological processes can be improved with the recent advancements in materials science and modern technology.


The Future of 'Self-Healing' Technologies

KOH Leng-Duei

    Recent years have witnessed significant advancement in 'self-healing' technologies. This is unlike a decade back, when 'self-healing' materials which are capable of restoring functionalities such as electrical conductivity or even optical (e.g., display) property after mechanical damage were practically non-existent. This editorial provides a quick introduction on reported 'self-healing' technologies,...


Honey incorporated antibacterial acellular dermal matrix for full-thickness wound healing

Archna Dhasmana*; Lubhan Singh; Partha Roy; Narayan Chandra Mishra

    Honey is a potent natural antibacterial remedy used for the treatment of chronic wounds. In this study, Honey incorporated Acellular Dermal Matrix (H-AGDM) was fabricated for accelerating the wound healing process. The physiochemical and morphological characterization of the fabricated matrix was performed by FTIR, FESEM, in vitro degradation study, and antibacterial activity.


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